My Breakthrough
Cell culture laboratory
What is a cell culture laboratory?
To study the molecules and genes that may be involved in the development and progression of breast cancer, the scientists grow many different types of cells in the laboratory.These are often derived from human breast cancer tumours and are called cell-lines. When stored correctly in liquid nitrogen, the cells can be maintained for many years, providing the scientists with a constant source of cells with which to work.
How are cells grown in the laboratory?
- The growth of cells in the laboratory is called 'cell culture'.
- The cells are grown in flasks that are often coated with special "glue" so that the cells can stick to the glassware.
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Each flask contains special growth liquid that contains all the nutrients which the cells need to survive. - The cells are grown in large incubators that are maintained at 37˚C. This is body temperature and is the ideal temperature for cell growth. The cells are protected from the external environment by double doors.
When the cells in one flask have grown so much that they cover the entire flask, they must be split into two or more new flasks. This process is called 'passaging' and needs to be carried out at least once a week. - Any research using these cells is carried out in special hoods called biological safety cabinets. The cabinet has a special filtered airflow, designed to prevent anything entering or leaving the work area. This provides an area inside the hood that is kept sterile so that the cells cannot become contaminated.
How is the cell culture laboratory being used in Breakthrough's Research?
Nearly all research groups in the Breakthrough Research Centre use the
cell culture facility.
- How breast cancer cells can move
- Whether new drugs are able to selectively destroy breast cancer cells
Also within "Research"
